اثرات افزودن ژل رویال به رقیق‌کننده تریس ـ زرده تخم مرغ بر فراسنجه‌های اسپرم قوچ افشاری پس از نگهداری‌ به صورت مایع

نوع مقاله: مقاله پژوهشی

نویسنده

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چکیده

مطالعه­ی حاضر با هدف بررسی اثرات محافظتی ژل رویال در رقیق­کننده تریس بر فراسنجه­های تحرک، زنده­مانی، ناهنجاری­های مورفولوژیک و تمامیت غشای پلاسمایی اسپرم قوچ در طول نگهداری به صورت مایع به مدت 7 روزانجام گرفت. تعداد 64 نمونه اسپرم از 4 راس قوچ افشاری با میانگین سن چهار سال با استفاده از واژن مصنوعی جمع­آوری شد.  پس از بررسی اولیه، نمونه­های منی با هم مخلوط شده و سپس با رقیق­کننده تریس ـ زرده بدون ژل رویال(شاهد) یا همراه با غلظت­های مختلف ژل رویال (5%، 3%،1%)رقیق گردید. تحرک کلی نمونه­های منی با استفاده از سیستم آنالیز کامپیوتری اسپرم (CASA) مورد بررسی قرار گرفت. فراسنجه­های مورفولوژی و زنده­مانی با روش رنگ­آمیزی ائوزین-نیگروزین و یکپارچگی غشا با آزمون تورم هیپواسموتیک (HOST) مورد ارزیابی قرار گرفتند. نمونه­های رقیق­شده به صورت مایع به مدت یک هفته در دمای 0C4 نگهداری شدند. نتایج نشان داد که درصد تحرک کل نمونه­های منی مایع پس از هفت روز نگهداری در دمای 0C4 در تیمار 3 درصد (86/2±25/51) به طور قابل توجهی از دیگر تیمارها بالاتر بود(05/0>P). درصد اسپرم­های دارای غشای سالم در نمونه­های رقیق­شده در رقیق­کننده­ی حاوی 3 درصد ژل رویال(96/2±53/46) به طور معنی­داری از گروه­ شاهد بالاتر بود(05/0>P). درصد اسپرم­های زنده­ درگروه 3 درصد(37/3±96/56) و 1 درصد(83/2±68/54) ژل رویال به طور معنی­داری بیشتر از گروه شاهد بود (05/0>P). به طور کلی، نتایج آزمایش حاضر نشان داد که افزودن 3% ژل رویال به رقیق کننده تریس موجب بهبود فراسنجه­های اسپرم قوچ در مدت ذخیره­سازی به صورت تازه گردید.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of adding royal jelly to Tris-egg yolk extender on Afshari ram sperm parameters after liquid storage of semen

نویسنده [English]

  • Amini, S., Masoumi, R., Rostami, B., Shahir, M.H. .
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چکیده [English]

The present study was conducted to evaluate the protective effects of royal jelly in Tris based extender on ram sperm parameters including motility, viability, morphology and plasma membrane integrity during 7 days liquid storage. 64 semen samples were collected with artificial vagina from 4 rams. After initial assessments, semen samples were mixed and then were diluted with Tris based extender having 0 (control), %1, %3 and %5 royal jelly. Motility was assessed with CASA system. Morphology and viability were assessed with Eosin-Negrosin staining method and plasma membrane integrity was assessed with HOST method. Diluted semen samples were kept in 37° for 1 hour and 4° C for 7 days. The results showed that motility percentage (51.25±2.86) in %3 royal jelly supplemented group was significantly higher than other treatment groups after 7 days’ liquid storage of semen (P< 0.05). The percentage of sperm cells with functional plasma membrane (46.53± 2.96) in %3 royal jelly supplemented group was significantly higher than control group (P< 0.05). The percentage of live sperm cells in %3 (56.96±3.37) and in 1% (54.68±2.83) royal jelly supplemented groups were higher than control group (P< 0.05). In conclusion, the results of present study showed that supplementation of %3 royal jelly to Tris based extender improves ram sperm parameters after 7 days’ liquid storage of semen.

کلیدواژه‌ها [English]

  • Ram
  • Semen
  • Royal jelly
  • Sperm
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