مطالعه اثرات حفاظتی عصاره سیاه‌دانه بر سمیت کبدی سیسپلاتین در موش صحرایی

نوع مقاله: مقاله پژوهشی

نویسنده

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چکیده

سیسپلاتین به­عنوان یک داروی ضدسرطان، در دوزهای بالا برای کبد سمّی می‌باشد. مشخص شده است که استرس اکسیداتیو در سمّیت سیسپلاتین دخیل است. با توجه به خواص آنتی­اکسیدانی سیاه‌دانه، این مطالعه برای ارزیابی اثرات محافظتی عصاره سیاه‌دانه در برابر سمیّت کبدی سیسپلاتین در موش­صحرایی انجام گردید.40 سر موش صحرایی نر ویستار به­طور تصادفی در چهار گروه مساوی تقسیم گردید. گروه 1 به­عنوان شاهد انتخاب شد. به گروه­های 2 و 4 عصاره سیاه‌دانه ( mg/kg250) به­مدت 15 روز متمادی گاواژ گردید. گروه­های 3 و 4 تک­دز سیسپلاتین (mg/kg  5/7) را در دهمین روز آزمایش به­روش داخل صفاقی دریافت کردند. در پایان، سطوح سرمی آنزیم‌های آسپارتات و آلانین آمینوترانسفراز و لاکتات دهیدروژناز و بیلی‌روبین تام، آلبومین و پروتئین تام اندازه­گیری شد. میزان مالون­دی­آلدئید و فعالیت آنزیم­های سوپراکسید دیسموتاز، کاتالاز، گلوتاتیون پراکسیداز و گلوتاتیون ردوکتاز نیز در هموژنات بافت کبد اندازه­گیری شد. برای ارزیابی آسیب شناسی بافتی، نمونه­هایی از  کبد جدا و مقاطع بافتی با استفاده از روش معمول رنگ­آمیزی هماتوکسیلین ـ ائوزین تهیه شد. در نهایت، یافته­های بیوشیمیایی با نتایج بافتی تطبیق داده شد.در گروه 4، عصاره سیاه‌دانه به‌طور معنی‌داری میزان افزایش یافته آنزیم‌های شاخص آسیب کبد و بیلی‌روبین تام را کاهش و سطوح کاهش یافته آلبومین و پروتئین تام سرم را به‌طور معنی‌داری افزایش داد. در این گروه، عصاره سیاه‌دانه به طور معنی­داری میزان پراکسیداسیون لیپیدی را کاهش و سطوح آنتی­اکسیدان­های کبد را افزایش داد. تغییرات بافتی همراستا با یافته­های بیوشیمیایی بود. تیمار با عصاره سیاه‌دانه، به­طور قابل توجهی مانع از بروز تغییرات پاتولوژیک در بافت کبد موش­های صحرایی شد و تنها آسیب قابل مشاهده، واکوئولاسیون خفیف سیتوپلاسم هپاتوسیت­ها به­خصوص در اطراف وریدچه مرکزی بود. نتایج این مطالعه نشان داد عصاره سیاه‌دانه با خواص آنتی‌اکسیدانی خود، کبد موش‌های صحرایی را در برابر سمّیت سیسپلاتین محافظت می­کند.

کلیدواژه‌ها


عنوان مقاله [English]

Protective effects of Black cumin (Nigella sativa Linn.) extract against cisplatin hepatotoxicity in rats

نویسنده [English]

  • Farjadifar, Y., Mohajeri, D., Kaffashi Elahi, R. .
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چکیده [English]

Cisplatin as an important anti-cancer drug is a potent hepatotoxicant. The aim of the present study was to evaluate the protective effect of ethanolic extract of Black cumin (Nigella sativa Linn.) against cisplatin-induced hepatotoxicity in the rats. 40 male Wistar rats were randomly allocated into 4 groups. Group 1 was used as control; groups 2 and 4 were orally treated with ethanolic extract of Black cumin (250 mg/kg) for 15 consecutive days. Groups 3 and 4 received a single intraperitoneal dose of cisplatin (7.5 mg/kg) on the 10th day of the experiment. At the end of experiment, serumic levels of aspartate and alanine transaminases, lactate dehydrogenase and total bilirubin, albumin and total proteins were assessed. Malondialdehyde, reduced glutathione and activities of superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase were assayed in liver homogenates. For histopathological evaluation, tissue samples were obtained from the livers and histologic sections were prepared by routine H&E staining method. Finally, the biochemical findings were matched with histopathological verification. In group 4, ethanolic extract of Black cumin significantly decreased the elevated levels of serum biomarkers of hepatic injury and total bilirubin, and significantly increased the reduced levels of serum albumin and total proteins. In this group, ethanolic extract of Black cumin significantly decreased the lipid peroxidation and elevated the decreased values of hepatic antioxidants. Histopathologically, the changes were in agreement with biochemical findings. Treatment with Black cumin extract considerably inhibited the pathological changes in the rat liver tissue and only visible damage was slight cytoplasmic vacuolation of hepatocytes especially around the central vein. The results of this study showed that, ethanolic extract of Black cumin, because of its anti-oxidant potential, exerts a protective effect against cisplatin induced hepatotoxicity in rats.

کلیدواژه‌ها [English]

  • Nigella sativa Linn
  • Cisplatin
  • Biochemical parameters
  • antioxidant
  • Histopathology
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