عنوان مقاله [English]
In these days press of hypertension and free radicals is the cause of disease in industrial
countries. Medicines by antihypertensive and antioxidant properties could inhibit these diseases.
In this study Shrimp byproducts were hydrolyzed with 5 different enzymes: Alcalase, Pepsin, α-
Chimotrypcin, Neutras and Flavourzyme. Angiotensin-I converting enzyme (ACE-Iinhibitory
activity), DPPH free radical scavenging activity (antioxidant properties) and amino acid
composition measured in the hydrolysates. With Alcalase ,the highest degree of hydrolysis (DH)
and nitrogen recovery was 64.2±1.4% and 75±1.6%, respectively. Antioxidant activity was
47.1±1.2% with Alcalase and 45.1±0.3% with α-Chimotrypcin. The maximum ACE-I inhibitory
activity was seen in the Alcalase hydrolysates and α-Chimotrypcin hydrolysates by
IC50=0.85±0.02 mg/ml and 0.9±0.04 mg/ml, respectively. ACE-Inhibition activity of
hydrolysates after ingestion by all enzymes was stable. Amino acid composition showed high
hydrophobic residues content. In conclusion, Shrimp by-products Protein hydrolysates produced
by Alcalase and α-Chimotrypcin have good ACE-Iinhibitory activity and antioxidant properties
and could be used in human nutrition as bioactive peptide.