عنوان مقاله [English]
Effects of Toxic in chemotherapy and radiotherapy are followed by many changes in male
reproductive system. Melatonin, one of the secretions of Epiphyses, known as an antioxidant.
The aim of this study is the investigation of protecting effect of Melatonin on the disordering of
spermatogenesis resulted in the Jemcitabin – therapy by analyzing the histology of testis.
Samples in this experimental intervened study are 48, 8weeks aged male wistar rat .Samples
randomly divided in 6 groups with 8 rats.
control group affected by normal Saline (IP)
Group 2,3Melatonin injected mono dosage (IP) in10, 20 mg/kg quantity
Group 4 Jemcitabin injected mono dosage (IP) in 50 mg/kg quantity
Group 5,6 Jemcitabin + Melatonin injected jemcitsbin in 50mg/kg and melatonin10,20mg/kg
(IP) in IP manner
14 days after therapy, the symbols made unconscious and segregate the rats`testis and
assessments was composed through the study of seminiferous tubules and counting of
spermatogenic and somatic cells.
Quantitative variables was evaluated and analyzed through non-parametric Kruskal-wellis by
the help of SPSS, supposing p<0.05is a criterion. the rats treated with jemcitabin compared with
control, showed significant decrease in assays and spermatogeneses quality p<0.01. quantitative
parameter of the seminiferous tubules in 4th group showed significant decrease compare with
control p<0.01.whereas melatonin in 6th group compared with4th group results in remarkable
natural seminiferous tubules parameter p<0.05. melatonin results significant increases in all
spermatogenic and somatic cells in 3th and 6th groups compared with 4th groups
p<0.05(improvement in spermatogeneses quality)and in 6th group neutralized side effects of
jemcitabin.the results of this study show that melatonin has protective effect on dameges which
induce by Jemcitabin. The mechanisms behind these actions are unknown at present however it
is possible to state preliminary that are mediated through reducing the oxidative damage.