عنوان مقاله [English]
Avian infectious bronchitis virus (IBV) is an important pathogen in poultry flocks, that
primarily induces respiratory diseases, but different IBV strains may also affect the other
tissues. The aim of this study was to investigate the tissue tropism of avian infectious bronchitis
virus strain 793/B in experimentally infected SPF chickens by RT-PCR and Nested-PCR assays.
In this study, tissue dissemination of Iranian isolate IR/773/2001(793/B) of IBV in different
organs of SPF chickens was studied. Forty two one-day-old SPF chicks were divided randomly
into two groups (21chicks per group). At the age of 12 days old the chicks in group1 were
inoculated intra-ocularly with 103EID50 of the 793/B isolate, and group2 was control group. The
samples from various tissues were collected at 2, 4, 6, 8, 10 and 12 days post-inoculation (PI).
The chicks of infected group exhibited depression at 2 to 4 days PI. The IBV virus was detected
in the cecal tonsils and cloaca from days 2-12 PI. Also virus detected in kidneys from days 4-10
PI, and in Bursa of fabricius from 4-12 days PI. In the trachea virus was detected on days 2 and
4 PI and in thymus samples virus was detected on days 6 and 8 PI. The positive sample from the
lungs only was at day 4 PI.
The results of this study indicated that the 793/B serotype of IBV cannot cause mortality, sever
clinical signs or gross lesions in infected chickens, but its replication in some tissues could
expose birds to other micro-organisms
قطعه جوجه یکروزه سن 12 روزگی جوجه های گروه یک با