عنوان مقاله [English]
نویسنده [English]چکیده [English]
The consumption of food containing staphylococcal enterotoxins is regularly identified as the
cause of intoxication. Enterotoxin A is considered as the most common toxin in staphylococcus–
related food poisoning. The purpose of this study was to determine the prevalence of S. aureus
in traditional white–brined cheese and distribution of enterotoxin A gene (sea) among them. A
total of 120 samples was examined and S. aureus was isolated from 11 (9.1%) of the samples.
According to the results, load of S. aureus was estimated from 1.5×101 to 8.6×104 cfu/g. No
sample was in the critical cell density of >105 cfu/g. From each sample, five suspected colonies
were confirmed by biochemical tests. S. aureus isolates were further identified based on 23S
rRNA, themonuclease and enterotoxin A genes using multiplex PCR. Based on multiplex PCR
results all 55 isolates were identified as S. aureus. The enterotoxin A gene (sea) was detected in
6 (10.9%) of the isolates. In conclusion, S. aureus and sea gene was found in traditional white–
brined cheese. It seems that if the favorable growth conditions are provided, S. aureus could
proliferate and produce enterotoxin and could be regarded as a potential risk for human health.