عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Salmonella is one of the authentic bacteria which cause illnesses, may exist in raw material and
food. The existence of these bacteria in food not only causes illnesses, but it also causes the
downfall of production quality and reduction of economic growth of the area and country. In
this study, 150 bulk raw milk samples were examined to comparison of PCR and conventional
culture for the identification of Salmonella in raw milk. Firstly raw milk was cultured and
examined through the conventional method; afterwards its supplementary procedures for
isolating Salmonella were carried out. Regarding to the results of the culture method, six
suspicious isolates were selected to carry out by PCR using invA gene. The results showed that
none of the isolates were salmonella. Secondly DNA extracted from raw milk and samples were
assessed utilizing the invA gene by PCR method. Regarding to the results 3 out of 150 examined
samples were positive. Totally 2 percent of all samples were contaminated with Salmonella.
The results of this study revealed that PCR is more potent than conventional culture methods to
identification of salmonella in raw milk.